Practical Histopathology in mouse models of human disease: Guides to Phenotyping the Genetically Altered mouse (see schedule of lectures below)

Course Organization:

1. Scope and Outline: The course is designed to guide investigators with the analysis of genetically altered mice. Lecture topics will emphazie use of histology, histochemistry, immunohistochemistry, in the interpretation of the histopathology of the various organ systems. Those who will benefit from attending the course will be graduate students, medical students, post-doctoral fellows and interested faculty. POSTDOCTORAL FELLOWS WHO ARE NOT AT UCSD MAY ATTEND THE COURSE. Lecture topics will include technical information and protocols, along with discussions on the various phenotyping methods available to help with the analysis of genetically altered mice, as compared with littermate controls. Identification of the different tissues and cell types in the body and the ability to detect abnormalities, will be the goal, thus enabling the optimal phenotypic analysis of genetically altered mice .
2. Teaching methods:

One two hour lecture/ demonstration per week for 8-10 weeks.

Lectures, demonstrations, multi media and computer support

3. Source Material

Textbooks:

a)      Pathology of the mouse. Ed. Robert R. Maronpot. Cache River Press

ISBN: 1-889899-02X

b)      di Fiores Atlas of Histology. Victor P. Eroschenko Lee and Febiger

c)      Histology. A text and Atlas. Ross, Romrell and Kaye. Williams and Wilkins

ISBN: 0-683-07369-9

d)      Human Histology. Stevens and Lowe. Mosby. ISBN: 0-7234-2485-3

e)      An Atlas of Histology. Zhang. Springer. ISBN: 0-387-94954-2

f)        Pathologic Basis of disease. Robbins,Kumar and Collins. Saunders.

ISBN: 0-7216-7335-X

g)      The Laboratory Mouse. Suckow, Danneman, Brayton. CRC Press

ISBN: 0-8493-0322-2

h)      A Color Atlas of Sectional Anatomy of the mouse. Iwaki, Yamashita, Hayakawa.

Braintree Scientific Inc. ISBN: 4-900659-58-4

i)        The Atlas of Mouse development. Kaufman. Academic Press.

ISBN 0-12-402035-6

j)        The House Mouse. Atlas of Embryonic development. Theiler. Springer-Verlag

ISBN: 0-387-05940-7

k)      Manipulating the mouse embryo, a laboratory manual. Hogan, Beddington, Costantini, Lacy. Cold Spring Harbor Laboratory Press ISBN: 0-87969-384-3

Evaluation:

1. Evaluation of Students:

Quiz at end of course

2. Evaluation of Course: Evaluation forms to be filled out by students at the end of each lecture:

a)      title of lecture

b)      information obtained met objectives, did not meet objectives

c)      lecture content: too detailed, just enough detail, not enough detail

d)      other comments

Classes

PATH 234/MED 234 / BIOM 238/ BGGN 234

Practical Histopathology in mouse models of human disease: Guides to Phenotyping the Genetically Altered mouse

Location: Basic Science Building: MDL lab-4
Time: Tuesday 1-3 pm

Course Director

Nissi Varki MD
Phone: 858-534-4933
Email: nvarki@ucsd.edu

Guest lecturers:

Dzung Le MD PhD, Department of Pathology
Michael Kalichman PhD, Department of Pathology
Lars Eckman MD, Department of Medicine
Maripot Corr MD, Department of Medicine
Mark Fuster MD, Department of Medicine
Kent Osborn DVM PhD, Department of Pathology

Microscopes and slides will be available at every session for individualized review. Where applicable, protocols will be available

Week	Date	Topic(s)	Instructor
1	Sept 15	Introduction to histology and histopathology (Cell injury/repair/death, inflammation, neoplasia, etc.) Histochemistry methods and protocols	Nissi Varki
2	Sept 22	Circulatory and Respiratory systems: Heart and blood vessels, skeletal muscle, smooth muscle Trachea and lungs: physiology /histopathology Hematopoietic system: Spleen, Thymus, Lymph nodes Bone Marrow: Markers for different cell types	Nissi Varki
3	Sept 29	Digestive system Part 1: Salivary Glands, Liver, Pancreas, Histopathology, Histochemistry, Overview of Immunohistochemistry methods	Nissi Varki
4	Oct 6	Hematopoietic system: Coagulation, chemistries, hematology, examples and protocols Genitourinary system: Kidney, Bladder	Dzung Le Nissi Varki
5	Oct 13	Responsible conduct of research Neural: Brain, cerebellum, Spinal cord, peripheral nerves Markers and examples of pathologies	Michael Kalichman Nissi Varki
6	Oct 20	Digestive system Part 2: Histopathology: Intestines: Esophagus, Stomach, Duodenum, Ileum, colon, cecum, appendix Mouse models of Inflammatory Bowel disease	Nissi Varki Lars Eckmann
7	Oct 27	Integument: Skin, Breast, Bone, Cartilage, Joints Mouse models of arthritis	Nissi Varki Maripat Corr
8	Nov 3	Genitourinary system: Female: Ovary, uterus, fallopian tube (human) Male: testis, epididymis, seminal vesicles, Prostate Mouse embryos, Placenta Mouse models of vascular integrity	Nissi Varki Mark Fuster
9	Nov 10	Endocrine: Pituitary, Thyroid, Parathyroid, Adrenal, Islets adipose tissue- fat, brown fat Review of organ systems	Nissi Varki
10	Nov 17	General approach to Pathologic Interpretation of Mouse Models	Kent Osborn Nissi Varki