Periodic Acid-Schiff Reagent (PAS)

Background:
Method:

• 1. Deparaffinize and hydrate
• 2. Periodic Acid- 5 minutes
• 3. MilliQ water Rinse
• 4. Immerse slides in Schiff Reagent- 15 minutes
• 5. Running Water- 10 minutes
• 6. Gill II Hematoxylin- 3 Dips (1 minute 30 seconds)
• 7. DI wash
• 8. Bluing Solution- 30 seconds
• 9. DI wash 10. Dehydrate and Clear
• 10. Coverslip

  Materials:

• 1. PAS kit: Sigma catalog#
• 2. Gill's hematoxylin