Periodic Acid-Schiff Reagent (PAS)

Background:
Method:

  • 1. Deparaffinize and hydrate
  • 2. Periodic Acid- 5 minutes
  • 3. MilliQ water Rinse
  • 4. Immerse slides in Schiff Reagent- 15 minutes
  • 5. Running Water- 10 minutes
  • 6. Gill II Hematoxylin- 3 Dips (1 minute 30 seconds)
  • 7. DI wash
  • 8. Bluing Solution- 30 seconds
  • 9. DI wash 10. Dehydrate and Clear
  • 10. Coverslip

    Materials:

  • 1. PAS kit: Sigma catalog#
  • 2. Gill's hematoxylin
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