Mayer's Mucicarmine

Background:
Method:

  • 1. Deparaffinize and hydrate to DI water
  • 2. Weigert's iron hematoxylin -7 min
  • 3. Brief tap water rinse
  • 4. Dilute acid alcohol Rinse
  • 5. Running Water - 10 min
  • 6. Mucicarmine working -- 30 min
  • 7. DI Rinse
  • 8. Metanil yellow - 1 min
  • 9. Dehydrate and clear
  • 10. Coverslip

Materials:v

    Mucicarmine Stock:
    Preheat water bath to boiling
    Mix in 500ml flask
    Shake well:

    1.0g Carmine 1.0g Aluminum Hydroxide 100.0 ml 50% Alcohol
    Add small amounts:
    .5gm Aluminum chloride (anhydrous)
    Boil flask 2-3 min
    Cool under cold tap

    Metanil Yellow (0.25%)
    Mix:

    .25g Metanil yellow 100.0 ml MQ water .25ml Acetic Acid
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