Trichrome (Masson)

Method:

• 1. Deparaffinize and hydrate
• 2. Bouins Solution: immerse slides for-: 1 hour in oven or overnight at 60 degrees
• 3. Running Water- let slides sit until yellow clears
• 4. Wiegert's Iron Hematoxylin- 10 minutes
• 5. Running Water- 5 minutes
• 6. Beibrich Scarlet-Acid Fushion- 5 minutes
• 7. DI Wash
• 8. Phosphotungstic-Phosphomolybdic Acid:5 minutes
• 9. Aniline Blue- 5 minutes
• 10. 1% Acetic Acid- 2 minutes
• 11. DI Rinse
• 12. Dehydrate, Clear and coverslip in fume hood

Materials:

• 1. Bouin's fixative Sigma Catalog #
• 2. Trichrome kit Sigma Catalog #HT-15
• 3. Phosphotungstic-Phosphomolybdic Acid replacements Sigma Catalog #HT15-2 and HT15-3