University of California San Diego Mouse Phenotype Service
Frequently Asked Questions

  1. It is highly recommended that you converse with a trained mouse histotechnologist for help with planning experiments that will need histology analyses. See Resources Tab.

  2. Optimum handling of mouse tissues: How does one decide whether the tissue is to be Frozen or FFPE (Formalin Fixed Paraffin Embedded)?

    Frozen sections are best used for immunohistochemistry assays, but morphologic detail is not well preserved usually. Frozen tissues that are correctly frozen with OCT compound, in a dry ice/isopentane slurry (see protocol) are cryo-sectioned in a CRYOSTAT.

    Paraffin Sections: Much better morphologic detail is obtained when examining standardly fixed, processed, stained PARAFFIN SECTIONS. It is best to label the cassettes yourself, using the indelible marker (NOT A SHARPIE, the ink of which is soluble in the solvents that are used to process the tissues into paraffin).

  3. For immunohistochemistry assays: Before embarking on time-consuming immunohistochemistry assays, it is important to confirm antibody reactivity using flow cytometry and Western Blots. This will also help the histotechnologist decide on concentration of anitbody to use when planning the immunohistochemistry assays.

    Also remember one can plan to use pellets of TISSUE CULTURE GROWN CELLS, which thus become important controls for immunohistochemistry experiments.

  4. Contact information for serum chemistry analyses at UC San Diego

  5. Contact information for analyses of muscle and peripheral nerve

  6. Mouse histology atlas

  7. Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

  8. Comparative human and mouse mammary gland and neoplasia

  9. Frozen section techniques

  10. Prepare frozen blocks

  11. Prepare formalin fixed paraffin embedded tissue blocks

  12. Mouse Necropsy protocols

  13. Jackson lab website

  14. Links to other useful information

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