Mayer's Mucicarmine

Background:
Method:

• 1. Deparaffinize and hydrate to DI water
• 2. Weigert's iron hematoxylin -7 min
• 3. Brief tap water rinse
• 4. Dilute acid alcohol Rinse
• 5. Running Water - 10 min
• 6. Mucicarmine working -- 30 min
• 7. DI Rinse
• 8. Metanil yellow - 1 min
• 9. Dehydrate and clear
• 10. Coverslip

Materials:v

Mucicarmine Stock:
Preheat water bath to boiling
Mix in 500ml flask
Shake well:


1.0g Carmine 1.0g Aluminum Hydroxide 100.0 ml 50% Alcohol

Add small amounts:
.5gm Aluminum chloride (anhydrous)
Boil flask 2-3 min
Cool under cold tap



Metanil Yellow (0.25%)
Mix:


.25g Metanil yellow 100.0 ml MQ water .25ml Acetic Acid